Tsib lub tshuab loj ntawm cov kua chromatograph yog raws li hauv qab no:
1. Kev txhaj tshuaj
Feem ntau, lub diaphragm txhaj tshuaj sampler lossis chav txhaj tshuaj siab yog siv los ua kom tiav qhov kev txhaj tshuaj, thiab qhov ntim txhaj tshuaj tas li.
Qhov no muaj txiaj ntsig zoo rau kev txhim kho kev rov ua dua ntawm cov qauv tshuaj ntsuam xyuas.
2. Infusion system
Lub kaw lus muaj peb ntu: lub twj tso kua mis siab, lub twj tso kua mis theem mobile, thiab lub gradienter. Lub siab dav dav ntawm lub twj tso kua mis siab yog 1.47 ~ 4.4X107Pa, thiab tus nqi ntws yog kho tau thiab ruaj khov. Thaum lub sij hawm high-pressure mobile dhau los ntawm chromatography kem, nws tuaj yeem txo cov nyhuv diffusion ntawm cov qauv hauv kem thiab ua kom nws lub zog ceev hauv kem. Qhov no muaj txiaj ntsig zoo rau kev txhim kho kev daws teeb meem, rov ua kom zoo dua qub, thiab tswj kev ua haujlwm lom neeg ntawm cov qauv. Txawb theem cia tsis raug thiab gradienters tuaj yeem hloov cov theem mobile raws li cov khoom ntawm cov theem nyob ruaj khov thiab cov qauv, suav nrog kev hloov cov polarity ntawm eluent, ion zog, pH nqi, los yog hloov mus rau lwm txoj kev.
3. Kev sib cais
Lub kaw lus suav nrog chromatography kab, txuas cov raj, thiab cov ntsuas kub.
Qhov ntev ntawm ib kab chromatographic feem ntau yog 10-50cm (yog tias xav tau ob qho sib xyaw ua ke, lub raj txuas tuaj yeem ntxiv ntawm ob), nrog rau txoj kab uas hla ntawm 2-5mm. Nws yog ua los ntawm cov hlau tsis zoo, tuab phab ntsa iav raj, titanium alloy, thiab lwm yam ntaub ntawv. Lub kem yog nruab nrog ib txoj kab uas hla ntawm 5-10 μ Ib theem ruaj khov nrog ib qho me me ntawm m (suav nrog cov matrix thiab cov kua ruaj khov), uas lub matrix yog tsim los ntawm cov neeg kho tshuab siab zog resin lossis silica gel, ob qho tib si. muaj cov yam ntxwv ntawm inertness (xws li qhov yooj yim tshem tawm ntawm silicate noob nyob rau saum npoo ntawm silica gel), porosity, thiab loj tshwj xeeb deg cheeb tsam, Tsis tas li ntawd, nws nto yog mechanically coated (zoo ib yam li kev npaj cov theem nyob ruaj ruaj hauv roj chromatography) los yog chemically ua ke nrog ntau yam noob (xws li phosphate, quaternary amine, hydroxymethyl, phenyl, amino lossis alkyl pawg ntawm ntau qhov ntev carbon chains) lossis ligands.
Yog li ntawd, cov khoom no nrog cov qauv sib txawv ruaj khov muaj qhov zoo xaiv. Piv txwv li, los ntawm kev sib txuas ntawm pea lectin (PSA) ntawm qhov ntxeem tau ntawm silica gel, glycoprotein tuaj yeem raug cais tawm ntawm fibroblasts. Tsis tas li ntawd, cov theem plasmid nyob ruaj khov yog me me, thiab lub txaj hauv kem yog yooj yim kom ua tiav lub xeev tsis sib xws thiab ntom ntom, uas tuaj yeem txo qhov eddy tam sim no diffusion nyhuv. Lub matrix muaj me me particle loj thiab ntiav micropores, thiab cov qauv muaj luv luv loj hloov nyob rau hauv microporous cheeb tsam. Cov no muaj txiaj ntsig zoo rau kev txo qis bandwidth thiab txhim kho kev daws teeb meem. Raws li kev soj ntsuam kev ua haujlwm ntawm kem, qhov me me ntawm cov matrix particle loj, qhov loj dua theoretical naj npawb N ntawm lub tais.
4. Kev kuaj xyuas qhov system
Muaj peb cov khoom siv feem ntau siv rau cov kua chromatography: UV ntes, qhov sib txawv ntawm qhov ntsuas qhov ntsuas qhov sib txawv, thiab fluorescence detector
UV ntes
Qhov ntsuas no tsim nyog rau kev kuaj cov qauv nrog kev ua haujlwm zoo rau ultraviolet (lossis pom lub teeb) lub teeb.
Nws cov yam ntxwv: ntau yam kev siv (xws li cov protein, nucleic acids, amino acids, nucleotides, peptides, cov tshuaj hormones, thiab lwm yam tuaj yeem siv tau); rhiab heev (nrhiav txwv ntawm 10-10g/mL); Wide linear ntau; Tsis rhiab rau qhov kub thiab txias hloov pauv; Muaj peev xwm ntes cov qauv eluted los ntawm gradient tov.
5. Cov ntaub ntawv ua haujlwm
Qhov system no tuaj yeem sau, khaws cia, tso saib, luam tawm, thiab ua cov ntaub ntawv xeem, ua kom muaj kev sib cais, kev npaj, lossis kev txheeb xyuas cov qauv.
Nyob rau hauv cov ntsiab lus, cov kua chromatograph muaj ib tug qauv system, ib tug infusion system, ib tug sib cais system, ib tug nrhiav kom tau, thiab cov ntaub ntawv ua system. Kev sib xyaw ua ke ntawm cov tshuab no ua rau qhov kev sim siab dua thiab ruaj khov.